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Aug 27 2008

Genebase Y-DNA STR Marker Table Version 3.4

Published by Genebaseteam under Y-DNA STR Markers

The following table outlines the Y-DNA STR markers available at Genebase and the corresponding motifs used for allele designation in Version 3.4.  Click here to view the conversion chart for Version 3.3 to Version 3.4.  Please note that Users from previous versions will be automatically converted to 3.4.

Y-STR marker

Motif

Comparison to NIST nomenclature
DYS19 [TAGA]3tagg[TAGA]n same motif
DYS385a/b [aagg]6-7[GAAA]n same motif
DYS388 [ATT]n same motif
DYS389 l/ll I:  [TCTG]3[TCTA]n      II: [TCTG]n[TCTA]nN28  [TCTG]3[TCTA]n       same motif
DYS390 (tcta)2[TCTG]n[TCTA]n[TCTG]n [TCTA]ntca(tcta)2 same motif
DYS391 (tctg)3[TCTA]n same motif
DYS392 [TAT]n same motif
DYS393 [AGAT]n same motif
DYS413a/b [CA]n ?
DYS426 [GTT]n same motif
DYS434 [CTAT]n same motif
DYS435 [TGGA]n same motif
DYS436 [GTT]n same motif
DYS437/DYS457 [TCTA]n[TCTG]n[TCTA]4 same motif
DYS438 [TTTTC]1[TTTTA]0-1[TTTTC]n same motif
DYS439 [GATA]n same motif
DYS441 [TTCC]n different motif
DYS442 [TATC]2[TGTC]3[TATC]n same motif
DYS444 [TAGA]n same motif
DYS445 [TTTA]n same motif
DYS446 [TCTCT]n same motif
DYS447 [TAATA]n[TAAAA]n[TAATA]n [TAAAA]n[TAATA]n same motif
DYS448 [AGAGAT]nN10[AGAGAT]3N14 [AGAGAT]n same motif
DYS449 [TTTC]n same motif
DYS450 [TTTTA]n same motif
DYS452 [TATAC]2[TGTAC]2[TATAC]n[CATAC]1[TATAC]1[CATAC]1  [TATAC]3-4[CATAC]0-2        [TATAC]0-3[CATAC]1[TATAC]3 same motif
DYS453 [AAAT]n same motif
DYS454 [AAAT]n same motif
DYS455 [AAAT]n same motif
DYS456 [AGAT]n same motif
DYS458 [GAAA]n same motif
DYS459a/b [TAAA]n same motif
DYS460 [ATAG]n same motif
DYS461 [TAGA]n[CAGA]n same motif
DYS462 [TATG]n same motif
DYS463 [AAAGG]n[AAGGG]n[AAGGA]2 same motif
DYS464a/b/c/d [CCTT]n same motif
DYS468 [TCC]n[TTC]n[(TTA)(TTC)]n[TTC]n ?
DYS472 [AAT]n ?
DYS481 [CTT]n same motif
DYS484 [AAT]n ?
DYS485 [TTA]n same motif
DYS487 [ATT]n ?
DYS490 [TTA]n same motif
DYS492 [ATT]n same motif
DYS494 [TAT]n ?
DYS495 [AAT]n same motif
DYS504 [TCCT]n same motif
DYS505 [TCCT]n same motif
DYS508 [TATC]n same motif
DYS511 [GATA]n ?
DYS518 [AAAG]n[GAAG]n[AAAG]n [GGAG]n[AAAG]n ?
DYS520 [ATAG]n[ATAC]n same motif
DYS522 [GATA]n same motif
DYS525 [TAGA]n same motif
DYS527a/b [GAAA]n[GGAA]n same motif
DYS531 [AAAT]n same motif
DYS532 [CTTT]n same motif
DYS533 [ATCT]n same motif
DYS534 [CTTT]n same motif
DYS537 [TCTA]n ?
DYS540 [TTAT]n same motif
DYS549 [AGAT] same motif
DYS556 [AATA]n same motif
DYS557 [TTTC]n same motif
DYS565 [TAAA]n same motif
DYS568 [AAAT]n ?
DYS570 [TTTC]n same motif
DYS572 [AAAT]n same motif
DYS575 [AAAT]n same motif
DYS576 [AAAG]n same motif
DYS578 [AAAT]n ?
DYS588 [TTGTA]n[TTGCA]n[TTGTA]n [TTGCA]n[TTGTA]n ?
DYS590 [TTTTG]n ?
DYS594 [TAAAA]n same motif
DYS607 [AAGG]n same motif
DYS612 [CCT]n[CTT]n[TCT]n[CCT]n[TCT]n ?
DYS614 [CTT]n[CCT]n[CTT]n[CCT]n[CTT]n [CCT]n[CTT]n[CCT]n[CTT]n[CCT]n [CTT]n[CCT]n[CTT]n[CCT]n[CTT]n ?
DYS617 [TTA]n ?
DYS626 [AAAG]n same motif
DYS632 [CATT]n same motif
DYS635/GATA C4 [TCTA]4[[TGTA]2[TCTA]2[TGTA]2 [TCTA]2[TGTA]0,2[TCTA]n same motif
DYS640 [AAAT]n ?
DYS641 [TAAA]n same motif
DYS643 [CTTTT]n same motif
DYS644 [TTTTA]n ?
DYS710 [AAAG]n[AG]n[AAAG]n same motif (different counting method)
DYS711 [CTT]n[CTTT]n[CTT]n ([CTC][CTT])compound[CTCCTTT]n[CTCCTA]n[CTT]n[CTC]n[CTT]n ?
DYS712 [AGAT]n[AGAC]n same motif
DYS713 [TCTT]ntc(TCTT)2tctgtcttttttctttct [CTTT]n(TCTT)5tcc(TTCT)2 (CTTTT)2ttta(TTTAT)4 ?
DYS714 [TTTCT]n[CTTCT]n[TTTCT]n [CTTCT]n[TTTCT]n same motif
DYS716 [CCATT]n different motif
DYS717 [TGTAT]n[TATTG]n[TACTG]n [TATTG]n different motif
DYS724a/b [AAAG]nN5[AAGG]n ?
GATA A10 [TAGA]n same motif
GATA H4 [TAGA]n same motif
YCAII a/b [CA]n same motif

 *Variable regions in each motif are indicated in blue.

*Motifs used for allele designation in Version 3.4 are compared to NIST nomenclature, and marked as “same” or “different” accordingly. 

*Question mark (?) indicates that NIST data is currently unavailable.

What is “NIST nomenclature”?

NIST stands for “National Institute of Standards and Technology”.  NIST is a non-regulatory federal agency within the U.S. Department of Commerce with the mission to promote U.S. innovation and industrial competitiveness by advancing measurement science, standards, and technology in ways that enhance economic security and improve our quality of life. 

NIST has compiled and maintained a Short Tandem Repeat DNA Internet Database called STRBase, a valuable STR information resource.  Click here to view the NIST standards for the most commonly tested Y-chromosome STR markers

To facilitate Y-DNA STR data comparisons between different laboratories and research groups, many laboratories choose to follow the standard NIST nomenclature to avoid differences in allele values due to different nomenclatures (motif and counting method) between different laboratories.  Most motifs in the NIST nomenclature follow the ISFG (International Society of Forensic Genetics) guidelines.  Click here to view the latest ISFG recommendations on the Y-STR nomenclature.

Jul 07 2008

Which markers are tested in the mtDNA SNP Haplogroup H Subclade Test Panel?

Published by Genebaseteam under mtDNA Tests

The mtDNA SNP Haplogroup H Subclade Test Panel examines 17 unique SNP markers in the coding region of the mtDNA.  These 17 SNP markers define up to 16 different subclades of Haplogroup H (Subclades 1 to 16).  The chart below lists the 17 SNP markers that are included in this panel and the Subclades that they define.

SNP Location Mutations Subclades of H
1438 A > G Excludes H2
2259 C > T H13
3010 G > A H1
3915 G > A H6
3936 C > T H12
3992 C > T H4
4310 A > G H9
4336 T > C H5a
4769 A > G Excludes H2a
4793 A > G H7
6253 T > C H15
6776 T > C H3
8448 T > C H11
10394 C > T H16
11377 G > A H14
13101 A > C H8
14470 T > A H10

The diagram below is a phylogenetic tree illustrating the various subclades of H that are known.  The diagram also shows where each of the 17 SNP fall in the tree.  Click here to download a detailed copy of the Haplogroup H Subclade Map

mtDNA SNP H-Refinement

Jun 21 2008

The mtDNA and its role in Ancestry: Part XVII (James “Earthquake McGoon” McGovern)

DNA Lesson Series: The mtDNA and its role in Ancestry
mtDNA Part I - mtDNA 101
mtDNA Part II - Facts about mtDNA
mtDNA Part III - mtDNA Structure
mtDNA Part IV - Ancestral Markers
mtDNA Part V - Detecting Mutations in the mtDNA
mtDNA Part VI - mtDNA Ancestral Markers
mtDNA Part VII - The Cambridge Reference Sequence
mtDNA Part VIII - mtDNA Test Types
mtDNA Part IX - mtDNA Haplogroup Determination
mtDNA Part X - mtDNA Subclades
mtDNA Part XI - mtDNA Haplogroup H
mtDNA Part XII - Subclades of mtDNA Haplogroup H
mtDNA Part XIII - Distribution of Subclades of H
mtDNA Part XIV - Descendents of Maria-Theresa
mtDNA Part XV - Luke the Evangelist
mtDNA Part XVI - Empress Feodorovna
mtDNA Part XVII - James “Earthquake McGoon” McGovern  <<– you are here

In the last three blogs, we have been discussing the topic of how anthropologists have used mtDNA to solve historical questions.  In particular, we have been focusing on the analysis of historical figures who may have belonged to Haplogroup H, one of the largest European Haplogroups.  We will wrap up our Haplogroup H series with this case about a World War II fighter ace and CIA pilot who died in combat in Vietnam. 

Case #4:  James “Earthquake McGoon” McGovern

Who was he?

James McGovern was a World War II fighter ace who died in a plane crash in Laos on May 6, 1954 when the Civil Air Transport plane that he was flying to provide ammunition to French troops was hit by groundfire.  McGovern and Buford (an American co-pilot) and two French Corporal Chiefs were killed instantly, making McGovern and Buford the first two Americans to die in combat in Vietnam. 

The source of his nickname:

At six feet and 260 pounds, McGovern was considered large for a fighter pilot, prompting his nickname “Earthquake McGoon” after the fierce and primitively charismatic (hulking hillbilly) wrestler from the popular Li’l Abner comic strip.

What were the anthropologists trying to find out? ie why was his DNA tested?

Between 1997 and 2002, multiple investigations of the site of incident were done by different teams (including joint US-Lao teams); however, only small fragments of aircraft wreckage were found, but no human burial sites were located.  In 2002, while investigating an unrelated crash near the Ban Sot area, the JTF-FA team discovered an old C-119 (the type of plan that McGovern was flying) propeller.  A few months later, human remains of a single individual were discovered from an unmarked grave, leading to speculation that the grave may have belonged to James McGovern.

The main purpose of the project was to determine whether the remains belonged to James McGovern.

Who’s Who in researching this project:

  • Irwin et al from Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, USA.
  • Holland et al from Joint POW/MIA Accounting Command - Central Identification Laboratory, Hawaii, USA

Top peer reviewed research publications for genetic identification of James McGovern:

This table lists the most significant papers for the James McGovern case and the DNA tests that were performed to identify his remains in peer reviewed journals.  Links are provided to access the original articles.

These papers provide the extent of what is known today about the DNA of James McGovern and provides answers to the question of whether the remains discovered in the grave belong to the James McGovern.

Name of Scientific Article Scientific Journal
DNA Identification of “Earthquake McGoon” 50 Years PostmortemIrwin et alArmed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, USA Click here to read abstracthttp://www.blackwell-synergy.com/doi/abs/10.1111/j.1556-4029.2007.00506.x J Forensic Sci.  2007 Sep; 52(5):  1115-8.

As further information becomes available, this table will be updated.

Solving the case: Collecting DNA samples

The first step in solving the case involved collecting DNA samples from the remains and locating living relatives of James McGovern for DNA testing.